PrimePCR™ SYBR® Green Assay: DUSP9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0051607


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PrimePCR™ PreAmp for SYBR® Green Assay: DUSP9, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: DUSP9, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044345
Assay Design:		exonic
Chromosome Location:		X:152915502-152915592question
Amplicon Length:		61
Splice Variants Targeted:		ENST00000547291 ENST00000308385 ENST00000279488 ENST00000357814 ENST00000580301 ENST00000429709 ENST00000450518 ENST00000392662 ENST00000495880 ENST00000296483 ENST00000225916 ENST00000370167 ENST00000433144 ENST00000342782 ENST00000371897 ENST00000371899 ENST00000356575 ENST00000440172 ENST00000422774 ENST00000242365 ENST00000290722 ENST00000375371 ENST00000567240 ENST00000563527 ENST00000564173 ENST00000267064 ENST00000347471 ENST00000550164 ENST00000394023 ENST00000595095 ENST00000601432 ENST

Gene Information

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases is expressed only in placenta kidney and fetal liver and is localized to the cytoplasm and nucleus. [provided by RefSeq Jul 2008]

Gene Symbol:		DUSP9
Gene Name:		dual specificity phosphatase 9
Aliases:		MKP-4, MKP4
RefSeq:		NC_000023.10 NT_167198.1
Ensembl:		ENSG00000130829
Entrez:		1852
Chromosome Mapping:		Xq28

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	36.010000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.