PrimePCR™ SYBR® Green Assay: ACLY, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051649

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ACLY, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACLY, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044387
Assay Design:   exonic
Chromosome Location:   17:40070048-40075148question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000319121 ENST00000352035 ENST00000590151 ENST00000537919 ENST00000393896 ENST00000592970 ENST00000353196 ENST00000297532 ENST00000482571 ENST00000272638 ENST00000569845 ENST00000564506 ENST00000567793

Gene Information

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product acetyl-CoA serves several important biosynthetic pathways including lipogenesis and cholesterogenesis. In nervous tissue ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ACLY
Gene Name:   ATP citrate lyase
Aliases:   ACL, ATPCL, CLATP
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000131473
Entrez:   47
Chromosome Mapping:   17q21.2

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