This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051736
PrimePCR™ PreAmp for SYBR® Green Assay: SLC14A2, Human
PrimePCR™ Template for SYBR® Green Assay: SLC14A2, Human
The protein encoded by this gene belongs to the urea transporter family. In mammalian cells urea is the chief end product of nitrogen catabolism and plays an important role in the urinary concentration mechanism. This protein is expressed in the inner medulla of the kidney and mediates rapid transepithelial urea transport across the inner medullary collecting duct. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.