PrimePCR™ SYBR® Green Assay: ELP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051785

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ELP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ELP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044523
Assay Design:   exonic
Chromosome Location:   8:28047197-28047336question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000521015 ENST00000256398 ENST00000524103 ENST00000523357 ENST00000517975 ENST00000380353 ENST00000537665 ENST00000542181

Gene Information

ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al. 2009 [PubMed 19185337]).[supplied by OMIM Apr 2009]

Gene Symbol:   ELP3
Gene Name:   elongation protein 3 homolog (S. cerevisiae)
Aliases:   FLJ10422, KAT9
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000134014
Entrez:   55140
Chromosome Mapping:   8p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.890000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download