PrimePCR™ SYBR® Green Assay: CTSL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051849

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CTSL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CTSL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044587
Assay Design:   exonic
Chromosome Location:   9:90344503-90344631question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000343150 ENST00000340342

Gene Information

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin as well as alpha-1 protease inhibitor a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia and in the renal tubular response to proteinuria. This protein which is a member of the peptidase C1 family is a dimer composed of disulfide-linked heavy and light chains both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Apr 2012]

Gene Symbol:   CTSL1
Gene Name:   cathepsin L1
Aliases:   CATL, CTSL, FLJ31037, MEP
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000135047
Entrez:   1514
Chromosome Mapping:   9q21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.900000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download