This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051998
PrimePCR™ PreAmp for SYBR® Green Assay: CTSL2, Human
PrimePCR™ Template for SYBR® Green Assay: CTSL2, Human
The protein encoded by this gene a member of the peptidase C1 family is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon mammary gland or peritumoral tissues suggesting a possible role for this gene in tumor processes. Alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.