PrimePCR™ SYBR® Green Assay: FXYD6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052044


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PrimePCR™ PreAmp for SYBR® Green Assay: FXYD6, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FXYD6, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044782
Assay Design:		exonic
Chromosome Location:		11:117708820-117708926question
Amplicon Length:		77
Splice Variants Targeted:		ENST00000260282 ENST00000527717 ENST00000526014 ENST00000524656 ENST00000584394 ENST00000529335 ENST00000540359 ENST00000539526

Gene Information

This gene encodes a member of the FXYD family of transmembrane proteins. This particular protein encodes phosphohippolin which likely affects the activity of NaK-ATPase. Multiple alternatively spliced transcript variants encoding the same protein have been described. Related pseudogenes have been identified on chromosomes 10 and X. Read-through transcripts have been observed between this locus and the downstream sodium/potassium-transporting ATPase subunit gamma (FXYD2 GeneID 486) locus.[provided by RefSeq Feb 2011]

Gene Symbol:		FXYD6
Gene Name:		FXYD domain containing ion transport regulator 6
Aliases:		Not Available
RefSeq:		NC_000011.9 NG_013071.1 NT_033899.8
Ensembl:		ENSG00000137726
Entrez:		53826
Chromosome Mapping:		11q23.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.900000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.