This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052050
PrimePCR™ PreAmp for SYBR® Green Assay: RTF1, Human
PrimePCR™ Template for SYBR® Green Assay: RTF1, Human
This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA. [provided by RefSeq Sep 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.