This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052222
PrimePCR™ PreAmp for SYBR® Green Assay: WDR61, Human
PrimePCR™ Template for SYBR® Green Assay: WDR61, Human
WDR61 is a subunit of the human PAF and SKI complexes which function in transcriptional regulation and are involved in events downstream of RNA synthesis such as RNA surveillance (Zhu et al. 2005 [PubMed 16024656]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.