PrimePCR™ SYBR® Green Assay: PMM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052236

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PMM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PMM2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044974
Assay Design:   exonic
Chromosome Location:   16:8904938-8905496question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000566983 ENST00000268261 ENST00000539622 ENST00000537352

Gene Information

The protein encoded by this gene catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Mutations in this gene have been shown to cause defects in glycoprotein biosynthesis which manifests as carbohydrate-deficient glycoprotein syndrome type I. [provided by RefSeq Jul 2008]

Gene Symbol:   PMM2
Gene Name:   phosphomannomutase 2
Aliases:   CDG1, CDG1a, CDGS, PMM 2
RefSeq:   NC_000016.9 NG_009209.1 NT_010393.16
Ensembl:   ENSG00000140650
Entrez:   5373
Chromosome Mapping:   16p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.990000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download