PrimePCR™ SYBR® Green Assay: GCSH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052244

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GCSH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GCSH, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044982
Assay Design:   exonic
Chromosome Location:   16:81116287-81116464question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000315467 ENST00000569885

Gene Information

Degradation of glycine is brought about by the glycine cleavage system which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase) H protein (a lipoic acid-containing protein) T protein (a tetrahydrofolate-requiring enzyme) and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants one protein-coding and the other probably not protein-codinghave been found for this gene. Also several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq Jan 2010]

Gene Symbol:   GCSH
Gene Name:   glycine cleavage system protein H (aminomethyl carrier)
Aliases:   GCE, NKH
RefSeq:   NC_000016.9 NG_016427.1 NT_010498.15
Ensembl:   ENSG00000140905
Entrez:   2653
Chromosome Mapping:   16q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download