PrimePCR™ SYBR® Green Assay: AZI1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052291

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AZI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AZI1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045029
Assay Design:   exonic
Chromosome Location:   17:79164481-79164709question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000563281 ENST00000374782 ENST00000269392 ENST00000570817 ENST00000573053 ENST00000450824 ENST00000575907 ENST00000571292 ENST00000532715 ENST00000374888 ENST00000290858 ENST00000412916 ENST00000323686 ENST00000330387 ENST00000456390 ENST00000332272 ENST00000390664 ENST00000378944 ENST00000314737 ENST00000592958 ENST00000587433 ENST00000587447 ENST00000007510 ENST00000396832 ENST00000359867

Gene Information

Description Not Available

Gene Symbol:   AZI1
Gene Name:   5-azacytidine induced 1
Aliases:   AZ1, Cep131, ZA1
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000141577
Entrez:   22994
Chromosome Mapping:   17q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.470000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download