This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052311
PrimePCR™ PreAmp for SYBR® Green Assay: IFNAR1, Human
PrimePCR™ Template for SYBR® Green Assay: IFNAR1, Human
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases which in turn phosphorylate several proteins including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.