PrimePCR™ SYBR® Green Assay: CBLC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0052314


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PrimePCR™ PreAmp for SYBR® Green Assay: CBLC, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CBLC, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045052
Assay Design:		exonic
Chromosome Location:		19:45281258-45281466question
Amplicon Length:		179
Splice Variants Targeted:		ENST00000270279 ENST00000341505

Gene Information

This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Feb 2012]

Gene Symbol:		CBLC
Gene Name:		Cas-Br-M (murine) ecotropic retroviral transforming sequence c
Aliases:		CBL-3, CBL-SL, RNF57
RefSeq:		NC_000019.9 NT_011109.16
Ensembl:		ENSG00000142273
Entrez:		23624
Chromosome Mapping:		19q13.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.320000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.