PrimePCR™ SYBR® Green Assay: SPIB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052323

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SPIB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SPIB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045062
Assay Design:   exonic
Chromosome Location:   19:50927000-50931422question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000378466 ENST00000239367 ENST00000546019 ENST00000434837 ENST00000505425 ENST00000297153 ENST00000303545 ENST00000270632 ENST00000596074 ENST00000595883 ENST00000439922 ENST00000599632 ENST00000207549 ENST00000412096

Gene Information

The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   SPIB
Gene Name:   Spi-B transcription factor (Spi-1/PU.1 related)
Aliases:   SPI-B
RefSeq:   NC_000019.9 NG_028026.1 NT_011109.16
Ensembl:   ENSG00000142539
Entrez:   6689
Chromosome Mapping:   19q13.3-q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 38.160000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download