PrimePCR™ SYBR® Green Assay: CELA2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052328

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CELA2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CELA2A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045067
Assay Design:   exonic
Chromosome Location:   1:15789288-15789905question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000359621 ENST00000375910

Gene Information

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1 2 2A 2B 3A and 3B. Like most of the human elastases elastase 2A is secreted from the pancreas as a zymogen. In other species elastase 2A has been shown to preferentially cleave proteins after leucine methionine and phenylalanine residues. [provided by RefSeq May 2009]

Gene Symbol:   CELA2A
Gene Name:   chymotrypsin-like elastase family, member 2A
Aliases:   ELA2A, PE-1
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000142615
Entrez:   63036
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.090000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download