PrimePCR™ SYBR® Green Assay: MARCH6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052533

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MARCH6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MARCH6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045272
Assay Design:   exonic
Chromosome Location:   5:10407311-10410342question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000449913 ENST00000503788 ENST00000274140 ENST00000510792

Gene Information

MARCH6 is an E3 ubiquitin (see MIM 191339) ligase that localizes to endoplasmic reticulum (ER) membranes (Hassink et al. 2005 [PubMed 15673284]).[supplied by OMIM Apr 2010]

Gene Symbol:   MARCH6
Gene Name:   membrane-associated ring finger (C3HC4) 6
Aliases:   DOA10, KIAA0597, MARCH-VI, RNF176, TEB4
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000145495
Entrez:   10299
Chromosome Mapping:   5p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.730000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download