PrimePCR™ SYBR® Green Assay: VIP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0052591


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PrimePCR™ PreAmp for SYBR® Green Assay: VIP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: VIP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045330
Assay Design:		exonic
Chromosome Location:		6:153080639-153080742question
Amplicon Length:		74
Splice Variants Targeted:		ENST00000367244 ENST00000367243 ENST00000431366

Gene Information

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility causes vasodilation increases glycogenolysis lowers arterial blood pressure and relaxes the smooth muscle of trachea stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:		VIP
Gene Name:		vasoactive intestinal peptide
Aliases:		MGC13587, PHM27
RefSeq:		NC_000006.11 NT_025741.15
Ensembl:		ENSG00000146469
Entrez:		7432
Chromosome Mapping:		6q25

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	35.680000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.