PrimePCR™ SYBR® Green Assay: ADM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052692

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ADM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADM, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045431
Assay Design:   exonic
Chromosome Location:   11:10327894-10328038question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000278175 ENST00000534464 ENST00000530439 ENST00000528655 ENST00000526492 ENST00000525063

Gene Information

Adrenomedullin a hypotensive peptide found in human pheochromocytoma consists of 52 amino acids has 1 intramolecular disulfide bond and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor called preproadrenomedullin is 185 amino acids long. By RNA-blot analysis human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns with the 5-prime flanking region containing TATA CAAT and GC boxes. There are also multiple binding sites for activator protein-2 and a cAMP-regulated enhancer element. [provided by RefSeq Jul 2008]

Gene Symbol:   ADM
Gene Name:   adrenomedullin
Aliases:   AM
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000148926
Entrez:   133
Chromosome Mapping:   11p15.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.950000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download