PrimePCR™ SYBR® Green Assay: ATP6V1G3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052775

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP6V1G3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP6V1G3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045514
Assay Design:   exonic
Chromosome Location:   1:198498286-198509794question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000367382 ENST00000309309 ENST00000489986 ENST00000367381 ENST00000281087 ENST00000340147 ENST00000573121 ENST00000571439 ENST00000575971

Gene Information

This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits two G subunits plus the C D E F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a c c' c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP6V1G3
Gene Name:   ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
Aliases:   ATP6G3, MGC119810, MGC119813, Vma10
RefSeq:   NC_000001.10 NT_004487.19 NW_003315907.1
Ensembl:   ENSG00000151418
Entrez:   127124
Chromosome Mapping:   1q31.3

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