PrimePCR™ SYBR® Green Assay: ZNF773, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052824

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045563
Assay Design:   exonic
Chromosome Location:   19:58016043-58016145question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000564906 ENST00000433976 ENST00000306502 ENST00000292562 ENST00000525191 ENST00000596831 ENST00000343280 ENST00000356584 ENST00000524372 ENST00000523882 ENST00000520540 ENST00000519310 ENST00000442920 ENST00000523312 ENST00000517598 ENST00000347466 ENST00000523138 ENST00000415379 ENST00000521754 ENST00000221735 ENST00000518999 ENST00000521137 ENST00000424930 ENST00000426954 ENST00000354197 ENST00000593916 ENST00000598770 ENST00000599847 ENST00000282292 ENST00000196489 ENST00000597700 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF773
Gene Name:   zinc finger protein 773
Aliases:   FLJ00301, MGC4728, ZNF419B
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000152439
Entrez:   374928
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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