PrimePCR™ SYBR® Green Assay: TRIM11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052898


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PrimePCR™ PreAmp for SYBR® Green Assay: TRIM11, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TRIM11, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045637
Assay Design:		exonic
Chromosome Location:		1:228589771-228593872question
Amplicon Length:		80
Splice Variants Targeted:		ENST00000588030 ENST00000589660 ENST00000588090 ENST00000591935 ENST00000586548 ENST00000589710 ENST00000588230 ENST00000413636 ENST00000586472 ENST00000589686 ENST00000587323 ENST00000320936 ENST00000587896 ENST00000589235 ENST00000591659 ENST00000585630 ENST00000586773 ENST00000444172 ENST00000493030 ENST00000284551 ENST00000602582 ENST00000366699

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified. [provided by RefSeq Jul 2008]

Gene Symbol:		TRIM11
Gene Name:		tripartite motif containing 11
Aliases:		BIA1, RNF92
RefSeq:		NC_000001.10 NT_167186.1
Ensembl:		ENSG00000154370
Entrez:		81559
Chromosome Mapping:		1q42.13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.420000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.