PrimePCR™ SYBR® Green Assay: CXXC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052916


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PrimePCR™ PreAmp for SYBR® Green Assay: CXXC1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CXXC1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045655
Assay Design:		exonic
Chromosome Location:		18:47812165-47812265question
Amplicon Length:		71
Splice Variants Targeted:		ENST00000412036 ENST00000285106 ENST00000589940 ENST00000591474 ENST00000589548 ENST00000586837

Gene Information

Proteins that contain a CXXC motif within their DNA-binding domain such as CXXC1 recognize CpG sequences and regulate gene expression (Carlone and Skalnik 2001 [PubMed 11604496]).[supplied by OMIM Mar 2008]

Gene Symbol:		CXXC1
Gene Name:		CXXC finger protein 1
Aliases:		2410002I16Rik, 5830420C16Rik, CFP1, CGBP, HsT2645, PCCX1, PHF18, SPP1, ZCGPC1, hCGBP
RefSeq:		NC_000018.9 NT_010966.14
Ensembl:		ENSG00000154832
Entrez:		30827
Chromosome Mapping:		18q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.770000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.