PrimePCR™ SYBR® Green Assay: VOPP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052920

List Price:    $183.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VOPP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VOPP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045659
Assay Design:   exonic
Chromosome Location:   7:55560103-55588779question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000341948 ENST00000380358 ENST00000358181 ENST00000330494 ENST00000285279 ENST00000433959 ENST00000428648 ENST00000428097 ENST00000418904 ENST00000453256 ENST00000427700 ENST00000455023 ENST00000414113 ENST00000417399 ENST00000452832 ENST00000545390 ENST00000268482 ENST00000256194 ENST00000527546 ENST00000537344 ENST00000342902 ENST00000379612

Gene Information

Description Not Available

Gene Symbol:   VOPP1
Gene Name:   vesicular, overexpressed in cancer, prosurvival protein 1
Aliases:   DKFZp564K0822, ECOP, FLJ20532, GASP
RefSeq:   NC_000007.13 NT_033968.6
Ensembl:   ENSG00000154978
Entrez:   81552
Chromosome Mapping:   7p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.510000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download