PrimePCR™ SYBR® Green Assay: EEF1A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052990


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PrimePCR™ PreAmp for SYBR® Green Assay: EEF1A1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EEF1A1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045729
Assay Design:		exonic
Chromosome Location:		6:74229061-74229187question
Amplicon Length:		97
Splice Variants Targeted:		ENST00000316292 ENST00000309268 ENST00000331523 ENST00000356303 ENST00000455918

Gene Information

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain placenta lung liver kidney and pancreas and the other isoform (alpha 2) is expressed in brain heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes some of which if not all represent different pseudogenes. [provided by RefSeq Jul 2008]

Gene Symbol:		EEF1A1
Gene Name:		eukaryotic translation elongation factor 1 alpha 1
Aliases:		CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1
RefSeq:		NC_000006.11 NT_007299.13
Ensembl:		ENSG00000156508
Entrez:		1915
Chromosome Mapping:		6q14.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.770000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.