This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053157
PrimePCR™ PreAmp for SYBR® Green Assay: TOR2A, Human
PrimePCR™ Template for SYBR® Green Assay: TOR2A, Human
This gene encodes a member of the AAA family of adenosine triphosphatases with similarity to Clp proteases and heat shock proteins. Alternative splicing at this locus results in the translation of multiple isoforms of the encoded protein some of which contain salusin peptides in the C-terminal region. These peptides may play roles in hypotension myocardial growth and the induction of mitogenesis and may also be involved in the pathogenesis of atherosclerosis. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.