PrimePCR™ SYBR® Green Assay: FGF11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053218

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FGF11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FGF11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045957
Assay Design:   exonic
Chromosome Location:   17:7346497-7346620question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000253686 ENST00000360423 ENST00000338971 ENST00000536184 ENST00000311916 ENST00000370228 ENST00000361060 ENST00000357308 ENST00000285071 ENST00000575235 ENST00000293829 ENST00000575331 ENST00000572907 ENST00000575398 ENST00000575082 ENST00000373509 ENST00000374259 ENST00000341558 ENST00000240123 ENST00000428103 ENST00000372912 ENST00000299432 ENST00000361577 ENST00000361462 ENST00000382233 ENST00000369026 ENST00000540590 ENST00000559004 ENST00000352105 ENST00000561243 ENST00000439576 ENST

Gene Information

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development cell growth morphogenesis tissue repair tumor growth and invasion. The function of this gene has not yet been determined. The expression pattern of the mouse homolog implies a role in nervous system development. [provided by RefSeq Jul 2008]

Gene Symbol:   FGF11
Gene Name:   fibroblast growth factor 11
Aliases:   FHF3, FLJ16061, MGC102953, MGC45269
RefSeq:   NC_000017.10 NG_008026.1 NT_010718.16
Ensembl:   ENSG00000161958
Entrez:   2256
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.160000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download