PrimePCR™ SYBR® Green Assay: FLYWCH2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053226

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FLYWCH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FLYWCH2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045965
Assay Design:   exonic
Chromosome Location:   16:2946502-2946616question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000273450 ENST00000472186 ENST00000452905 ENST00000393434 ENST00000393431 ENST00000356083 ENST00000418432 ENST00000292377 ENST00000446533 ENST00000569899 ENST00000427939 ENST00000317716 ENST00000478080 ENST00000542731 ENST00000343407 ENST00000396958 ENST00000293981 ENST00000573965 ENST00000572006 ENST00000253928 ENST00000416288 ENST00000573525 ENST00000399667 ENST00000256190 ENST00000266674 ENST00000536515 ENST00000540815 ENST00000428977 ENST00000429976 ENST00000301807 ENST00000574946 ENST

Gene Information

Description Not Available

Gene Symbol:   FLYWCH2
Gene Name:   FLYWCH family member 2
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000162076
Entrez:   114984
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995900
y-intercept 39.000000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download