PrimePCR™ SYBR® Green Assay: FAIM3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053284


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PrimePCR™ PreAmp for SYBR® Green Assay: FAIM3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FAIM3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0046023
Assay Design:		exonic
Chromosome Location:		1:207086305-207087113question
Amplicon Length:		63
Splice Variants Targeted:		ENST00000367091 ENST00000442471 ENST00000525793 ENST00000529560 ENST00000420007 ENST00000559476 ENST00000446923

Gene Information

Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM (see MIM 147020) (Kubagawa et al. 2009 [PubMed 19858324]; Shima et al. 2010 [PubMed 20042454]).[supplied by OMIM Jul 2010]

Gene Symbol:		FAIM3
Gene Name:		Fas apoptotic inhibitory molecule 3
Aliases:		FCMR, TOSO
RefSeq:		NC_000001.10 NT_167186.1
Ensembl:		ENSG00000162894
Entrez:		9214
Chromosome Mapping:		1q32.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.840000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.