PrimePCR™ SYBR® Green Assay: PPM1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053368

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPM1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPM1L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046107
Assay Design:   exonic
Chromosome Location:   3:160679561-160679684question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000497343 ENST00000498165 ENST00000464260 ENST00000295839

Gene Information

PPM1L or PP2CE belongs to the PP2C group of serine/threonine phosphatases which are distinguished from other phosphatases by their structure absolute requirement for Mg(2+) or Mn(2+) and insensitivity to okadaic acid. PP2Cs regulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extracellular stimuli (Jin et al. 2004 [PubMed 15560375]).[supplied by OMIM Apr 2008]

Gene Symbol:   PPM1L
Gene Name:   protein phosphatase, Mg2+/Mn2+ dependent, 1L
Aliases:   MGC132545, MGC132547, PP2C-epsilon, PP2CE, PPM1-LIKE
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000163590
Entrez:   151742
Chromosome Mapping:   3q26.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.390000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download