PrimePCR™ SYBR® Green Assay: GPR85, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053494

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GPR85, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPR85, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046233
Assay Design:   exonic
Chromosome Location:   7:112724645-112724742question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000501255 ENST00000438062 ENST00000449735 ENST00000449591 ENST00000297146 ENST00000424100

Gene Information

Members of the G protein-coupled receptor (GPCR) family such as GPR85 have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters hormones or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins or G proteins (Matsumoto et al. 2000 [PubMed 10833454]).[supplied by OMIM Aug 2008]

Gene Symbol:   GPR85
Gene Name:   G protein-coupled receptor 85
Aliases:   FLJ50993, FLJ51159, FLJ99433, SREB, SREB2
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000164604
Entrez:   54329
Chromosome Mapping:   7q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download