PrimePCR™ SYBR® Green Assay: ANKRD2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0053596


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PrimePCR™ PreAmp for SYBR® Green Assay: ANKRD2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ANKRD2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0046335
Assay Design:		exonic
Chromosome Location:		10:99342372-99343341question
Amplicon Length:		67
Splice Variants Targeted:		ENST00000298808 ENST00000307518 ENST00000455090 ENST00000370655

Gene Information

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress injury and hypertrophy (Miller et al. 2003 [PubMed 14583192]).[supplied by OMIM Mar 2008]

Gene Symbol:		ANKRD2
Gene Name:		ankyrin repeat domain 2 (stretch responsive muscle)
Aliases:		ARPP, MGC104314
RefSeq:		NC_000010.10 NG_027922.1 NT_030059.13
Ensembl:		ENSG00000165887
Entrez:		26287
Chromosome Mapping:		10q23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998200
y-intercept	36.380000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.