PrimePCR™ SYBR® Green Assay: TRIM66, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053643

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046382
Assay Design:   exonic
Chromosome Location:   11:8643254-8643362question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000402157 ENST00000530502 ENST00000299550

Gene Information

Description Not Available

Gene Symbol:   TRIM66
Gene Name:   tripartite motif containing 66
Aliases:   C11orf29, FLJ10046, TIF1D, TIF1DELTA
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000166436
Entrez:   9866
Chromosome Mapping:   11p15.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.730000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download