PrimePCR™ SYBR® Green Assay: TMEM135, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053656

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TMEM135, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TMEM135, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046395
Assay Design:   exonic
Chromosome Location:   11:86749141-86778745question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000245663 ENST00000302995 ENST00000395104 ENST00000341547 ENST00000377499 ENST00000526202 ENST00000340353 ENST00000526733 ENST00000525018 ENST00000305494 ENST00000355734 ENST00000535167

Gene Information

Description Not Available

Gene Symbol:   TMEM135
Gene Name:   transmembrane protein 135
Aliases:   DKFZp686I1974, FLJ22104
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000166575
Entrez:   65084
Chromosome Mapping:   11q14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.870000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download