PrimePCR™ SYBR® Green Assay: TTYH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053731

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TTYH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TTYH1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046470
Assay Design:   exonic
Chromosome Location:   19:54930360-54932457question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000393976 ENST00000361545 ENST00000223398 ENST00000423529 ENST00000301194 ENST00000376530 ENST00000445095 ENST00000376531 ENST00000391739 ENST00000219611 ENST00000296511 ENST00000501272 ENST00000515017 ENST00000368171 ENST00000297151 ENST00000257863 ENST00000550311 ENST00000393229 ENST00000360670 ENST00000393228 ENST00000372179 ENST00000302262 ENST00000390015 ENST00000575290 ENST00000575893 ENST00000574216 ENST00000571818 ENST00000575814 ENST00000570708 ENST00000576642 ENST00000575157 ENST

Gene Information

This gene encodes a member of the tweety family of proteins. Members of this family function as chloride anion channels. The encoded protein functions as a calcium(2+)-independent volume-sensitive large conductance chloride(-) channel. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:   TTYH1
Gene Name:   tweety homolog 1 (Drosophila)
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000167614
Entrez:   57348
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 37.140000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download