PrimePCR™ SYBR® Green Assay: UBXN6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053741

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PrimePCR™ PreAmp for SYBR® Green Assay: UBXN6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: UBXN6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046480
Assay Design:   exonic
Chromosome Location:   19:4445620-4446181question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000400829 ENST00000400827 ENST00000301332 ENST00000332312 ENST00000326321 ENST00000270442 ENST00000591919 ENST00000301281 ENST00000394765 ENST00000590466 ENST00000570673 ENST00000576113 ENST00000573879 ENST00000576676 ENST00000575378 ENST00000572162 ENST00000576619 ENST00000576605 ENST00000574156

Gene Information

Description Not Available

Gene Symbol:   UBXN6
Gene Name:   UBX domain protein 6
Aliases:   DKFZp667D109, FLJ00394, UBXD1, UBXDC2
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000167671
Entrez:   80700
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.020000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download