PrimePCR™ SYBR® Green Assay: KIF5C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053796

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIF5C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIF5C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046535
Assay Design:   exonic
Chromosome Location:   2:149806826-149806970question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000435030 ENST00000450621 ENST00000414838 ENST00000397413 ENST00000455537 ENST00000286452 ENST00000576072 ENST00000575453 ENST00000573697 ENST00000574197

Gene Information

Description Not Available

Gene Symbol:   KIF5C
Gene Name:   kinesin family member 5C
Aliases:   FLJ44735, KIAA0531, KINN, MGC111478, NKHC, NKHC-2, NKHC2
RefSeq:   NC_000002.11 NT_005403.17 NT_022135.16
Ensembl:   ENSG00000168280
Entrez:   3800
Chromosome Mapping:   2q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.680000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download