PrimePCR™ SYBR® Green Assay: WNT10B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053928


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PrimePCR™ PreAmp for SYBR® Green Assay: WNT10B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: WNT10B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0046667
Assay Design:		exonic
Chromosome Location:		12:49363959-49364251question
Amplicon Length:		159
Splice Variants Targeted:		ENST00000393989 ENST00000246639 ENST00000393986 ENST00000328119 ENST00000377264 ENST00000421419 ENST00000361203 ENST00000439203 ENST00000370754 ENST00000370769 ENST00000446842 ENST00000312431 ENST00000389641 ENST00000392343 ENST00000392347 ENST00000563588 ENST00000415757 ENST00000389232 ENST00000368069 ENST00000241704 ENST00000541260 ENST00000543610 ENST00000545825 ENST00000567020 ENST00000293970 ENST00000564879 ENST00000304031 ENST00000399275 ENST00000350148 ENST00000379826 ENST00000264607 ENST

Gene Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member WNT1 in the chromosome 12q13 region. [provided by RefSeq Jul 2008]

Gene Symbol:		WNT10B
Gene Name:		wingless-type MMTV integration site family, member 10B
Aliases:		SHFM6, WNT-12
RefSeq:		NC_000012.11 NG_023347.1 NT_029419.12
Ensembl:		ENSG00000169884
Entrez:		7480
Chromosome Mapping:		12q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.740000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.