PrimePCR™ SYBR® Green Assay: GPR137, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054228

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GPR137, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPR137, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046967
Assay Design:   exonic
Chromosome Location:   11:64054268-64054444question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000546139 ENST00000411458 ENST00000536667 ENST00000539833 ENST00000377702 ENST00000535675 ENST00000543383 ENST00000538032 ENST00000438980 ENST00000313074 ENST00000542190 ENST00000541952 ENST00000539851

Gene Information

Description Not Available

Gene Symbol:   GPR137
Gene Name:   G protein-coupled receptor 137
Aliases:   C11orf4, GPR137A, TM7SF1L1
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000173264
Entrez:   56834
Chromosome Mapping:   11cen-q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download