PrimePCR™ SYBR® Green Assay: MST1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054250


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PrimePCR™ PreAmp for SYBR® Green Assay: MST1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MST1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0046989
Assay Design:		exonic
Chromosome Location:		3:49724607-49724710question
Amplicon Length:		74
Splice Variants Targeted:		ENST00000221347 ENST00000303746 ENST00000575885 ENST00000571007 ENST00000319500 ENST00000575769 ENST00000308354 ENST00000397761 ENST00000409141 ENST00000265810 ENST00000409417 ENST00000397760 ENST00000339162 ENST00000537592 ENST00000575389 ENST00000536229 ENST00000313234 ENST00000394815 ENST00000576809 ENST00000572106 ENST00000545762 ENST00000383728 ENST00000449682

Gene Information

The protein encoded by this gene contains four kringle domains and a serine protease domain similar to that found in hepatic growth factor. Despite the presence of the serine protease domain the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. [provided by RefSeq Jan 2010]

Gene Symbol:		MST1
Gene Name:		macrophage stimulating 1 (hepatocyte growth factor-like)
Aliases:		D3F15S2, DNF15S2, HGFL, MSP, NF15S2
RefSeq:		NC_000003.11 NG_011438.1 NG_011603.1 NG_016454.1 NT_022517.18
Ensembl:		ENSG00000173531
Entrez:		4485
Chromosome Mapping:		3p21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998400
y-intercept	36.790000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.