PrimePCR™ SYBR® Green Assay: GPRC6A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054256

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GPRC6A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPRC6A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046995
Assay Design:   exonic
Chromosome Location:   6:117128203-117128303question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000310357 ENST00000368549 ENST00000530250

Gene Information

Members of family C of the G protein-coupled receptor (GPCR) superfamily such as GPRC6A are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C including GPRC6A also have a long N-terminal domain (summary by Pi et al. 2005 [PubMed 16199532]).[supplied by OMIM Nov 2010]

Gene Symbol:   GPRC6A
Gene Name:   G protein-coupled receptor, family C, group 6, member A
Aliases:   GPCR, bA86F4.3
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000173612
Entrez:   222545
Chromosome Mapping:   6q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.370000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download