PrimePCR™ SYBR® Green Assay: SLC29A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0054319


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC29A2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC29A2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0047058
Assay Design:		exonic
Chromosome Location:		11:66130574-66130691question
Amplicon Length:		88
Splice Variants Targeted:		ENST00000311161 ENST00000357440 ENST00000544554 ENST00000546034

Gene Information

The uptake of nucleosides by transporters such as SLC29A2 is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al. 1997 [PubMed 9396714]).[supplied by OMIM Nov 2008]

Gene Symbol:		SLC29A2
Gene Name:		solute carrier family 29 (nucleoside transporters), member 2
Aliases:		DER12, ENT2, HNP36
RefSeq:		NC_000011.9 NT_167190.1
Ensembl:		ENSG00000174669
Entrez:		3177
Chromosome Mapping:		11q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	36.360000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.