PrimePCR™ SYBR® Green Assay: CSRP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054354

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PrimePCR™ PreAmp for SYBR® Green Assay: CSRP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CSRP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047093
Assay Design:   exonic
Chromosome Location:   12:77252633-77252759question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000311083 ENST00000552330 ENST00000546966

Gene Information

CSRP2 is a member of the CSRP family of genes encoding a group of LIM domain proteins which may be involved in regulatory processes important for development and cellular differentiation. CRP2 contains two copies of the cysteine-rich amino acid sequence motif (LIM) with putative zinc-binding activity and may be involved in regulating ordered cell growth. Other genes in the family include CSRP1 and CSRP3. [provided by RefSeq Jul 2008]

Gene Symbol:   CSRP2
Gene Name:   cysteine and glycine-rich protein 2
Aliases:   CRP2, LMO5, SmLIM
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000175183
Entrez:   1466
Chromosome Mapping:   12q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.390000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download