PrimePCR™ SYBR® Green Assay: KCNJ10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0054531


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PrimePCR™ PreAmp for SYBR® Green Assay: KCNJ10, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: KCNJ10, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0047271
Assay Design:		exonic
Chromosome Location:		1:160010519-160010652question
Amplicon Length:		104
Splice Variants Targeted:		ENST00000368089

Gene Information

This gene encodes a member of the inward rectifier-type potassium channel family characterized by having a greater tendency to allow potassium to flow into rather than out of a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq Jul 2008]

Gene Symbol:		KCNJ10
Gene Name:		potassium inwardly-rectifying channel, subfamily J, member 10
Aliases:		BIRK-10, KCNJ13-PEN, KIR1.2, KIR4.1, SESAME
RefSeq:		NC_000001.10 NG_016411.1 NT_004487.19
Ensembl:		ENSG00000177807
Entrez:		3766
Chromosome Mapping:		1q23.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	36.370000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.