PrimePCR™ SYBR® Green Assay: GRINA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054588

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GRINA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GRINA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047328
Assay Design:   exonic
Chromosome Location:   8:145066424-145066539question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000529301 ENST00000395068 ENST00000534791 ENST00000533044 ENST00000527194 ENST00000313269 ENST00000562033 ENST00000569564 ENST00000569797 ENST00000569361 ENST00000566404 ENST00000269218 ENST00000580732 ENST00000424526 ENST00000400483 ENST00000431264 ENST00000370507 ENST00000217159

Gene Information

Description Not Available

Gene Symbol:   GRINA
Gene Name:   glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding)
Aliases:   HNRGW, LFG1, MGC99687, NMDARA1, TMBIM3
RefSeq:   NC_000008.10 NT_008046.16 NW_003315923.1
Ensembl:   ENSG00000178719
Entrez:   2907
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.210000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download