PrimePCR™ SYBR® Green Assay: MED14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054668

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MED14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MED14, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047408
Assay Design:   exonic
Chromosome Location:   X:40513618-40513760question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000324817 ENST00000416199 ENST00000433003

Gene Information

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex which along with TFIID is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq Jul 2008]

Gene Symbol:   MED14
Gene Name:   mediator complex subunit 14
Aliases:   CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, MGC104513, RGR1, TRAP170
RefSeq:   NC_000023.10 NG_016325.1 NG_016376.1 NT_079573.4
Ensembl:   ENSG00000180182
Entrez:   9282
Chromosome Mapping:   Xp11.4

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