PrimePCR™ SYBR® Green Assay: TNRC18, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054767

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TNRC18, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TNRC18, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047507
Assay Design:   exonic
Chromosome Location:   7:5434096-5460706question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000432382 ENST00000348956 ENST00000373736 ENST00000263693 ENST00000398657 ENST00000359206 ENST00000265723 ENST00000453593 ENST00000358400 ENST00000545634 ENST00000430216 ENST00000414810 ENST00000454820 ENST00000442688 ENST00000216267 ENST00000404034 ENST00000404760 ENST00000542442 ENST00000342989 ENST00000457780 ENST00000373624 ENST00000377551 ENST00000377559 ENST00000409571 ENST00000265459 ENST00000399537 ENST00000434361 ENST00000399434 ENST00000430969 ENST00000448169 ENST00000437726 ENST

Gene Information

Description Not Available

Gene Symbol:   TNRC18
Gene Name:   trinucleotide repeat containing 18
Aliases:   CAGL79, KIAA1856, TNRC18A
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000182095
Entrez:   84629
Chromosome Mapping:   7p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.540000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download