PrimePCR™ SYBR® Green Assay: ATP6AP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0054780


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PrimePCR™ PreAmp for SYBR® Green Assay: ATP6AP2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ATP6AP2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0047520
Assay Design:		exonic
Chromosome Location:		X:40457984-40458988question
Amplicon Length:		118
Splice Variants Targeted:		ENST00000378438 ENST00000447485 ENST00000423649 ENST00000535539 ENST00000535777 ENST00000544975

Gene Information

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation secondary active transport acidification of intracellular compartments and cellular pH homeostasis. There are three classes of ATPases- F P and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq Jul 2008]

Gene Symbol:		ATP6AP2
Gene Name:		ATPase, H+ transporting, lysosomal accessory protein 2
Aliases:		APT6M8-9, ATP6IP2, ATP6M8-9, ELDF10, M8-9, MGC99577, MRXE, MSTP009, XMRE
RefSeq:		NC_000023.10 NG_008874.1 NT_079573.4
Ensembl:		ENSG00000182220
Entrez:		10159
Chromosome Mapping:		Xp11.4

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.610000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.