PrimePCR™ SYBR® Green Assay: TREX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0054858

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TREX2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TREX2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047598
Assay Design:   exonic
Chromosome Location:   X:152710265-152710375question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000263650 ENST00000389989 ENST00000525498 ENST00000348039 ENST00000542243 ENST00000393862 ENST00000330912 ENST00000338525 ENST00000334497 ENST00000370232 ENST00000402951 ENST00000414588 ENST00000370231 ENST00000344302 ENST00000222254 ENST00000600040 ENST00000597970 ENST00000596566 ENST00000595172 ENST00000595261 ENST00000594277 ENST00000595871 ENST00000596889

Gene Information

This gene encodes a protein with 3' exonuclease activity. Enzymes with this activity are involved in DNA replication repair and recombination. Similarity to an E. coli protein suggests that this enzyme may be a subunit of DNA polymerase III which does not have intrinsic exonuclease activity. [provided by RefSeq Jul 2008]

Gene Symbol:   TREX2
Gene Name:   three prime repair exonuclease 2
Aliases:   Not Available
RefSeq:   NC_000023.10 NG_012688.1 NT_167198.1
Ensembl:   ENSG00000183479
Entrez:   11219
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.040000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download