PrimePCR™ SYBR® Green Assay: SNAI3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055021

List Price:    $179.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: SNAI3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SNAI3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047761
Assay Design:   exonic
Chromosome Location:   16:88744098-88744189question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000381418 ENST00000312841 ENST00000367201 ENST00000367202 ENST00000367199 ENST00000367198 ENST00000332281

Gene Information

SNAI3 is a member of the SNAIL gene family named for the Drosophila snail gene which plays roles in mesodermal formation during embryogenesis (Katoh and Katoh 2003 [PubMed 12579345]).[supplied by OMIM Apr 2009]

Gene Symbol:   SNAI3
Gene Name:   snail homolog 3 (Drosophila)
Aliases:   MGC129606, SMUC, SNAIL3, ZNF293, Zfp293
RefSeq:   NC_000016.9 NT_010542.15
Ensembl:   ENSG00000185669
Entrez:   333929
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.560000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download